Primesep 200 separates the very hydrophilic polyamines, 1,3-diaminopropane, N-(3-aminopropyl)

-1,3-diaminepropane, N,N-bis-(3-aminopropyl)-1,3-diaminepropane. The retention and resolution are affected

by either a TFA concentration gradient, or a pH gradient from pH 4 to 3. This flexibility is obtained by simple

mobile phases of water, acetonitrile (MeCN, ACN), ammonium formate, ammonium acetate or trifluoracetic

acid (TFA) with evaporative light scattering detection (ELSD).

Application Name : HPLC Separation of Polyamines

Column Name : Primesep 200 Columns

Analytes : 1,3-Propanediamine, N,N＇- Bis(3-aminopropyl)-1,3-propanediamine,N-(3-Aminopropyl)-1,3-propanediamine