Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm.

Application Name : Hydrophobic and Hydrophilic Compound Separation

Column Name : Primesep 100 Columns

Analytes : Toluene,Benzoic acid,Cystine,2-Methylcysteine,L-Cysteine,2-2-Dimethylcystine,L-Cystine